. 2015 Mar 21;96(4):887–900. doi: 10.1111/mmi.12979

Table 1.

Effects of mutations in TbAT 1 amino acids 286 and 380 on kinetic parameters of adenosine transport

	TbAT1‐WT	TbAT1^L380P	TbAT1^N286S+L380P
K_m (μM)	0.82 ± 0.25	0.69 ± 0.13	1.02 ± 0.29
V_max (pmol(10⁷ cells)⁻¹ s⁻¹)	0.35 ± 0.07	0.12 ± 0.01^a	0.027 ± 0.008^b
V_max/K_m	0.43	0.17	0.026

V_max and K_m values for TbAT1‐mediated [³H]‐adenosine transport were determined in the presence of 1 mM inosine, using 0.1 μM radiolabel and 10 s incubations to determine the initial rates of transport. The data shown are the average and standard error of the mean of four independent experiments, each performed in triplicate, in which the parameters for all three strains was determined in parallel, using the same solutions and conditions.

P < 0.02.

P < 0.01 (unpaired Student's t‐test, relative to the wild‐type allele of TbAT1).