Figure 1.

The diterpene chemoscape. A) Levels of diterpenes accumulating in the N. benthamiana/Agrobacterium system (n=3). B) GC–MS chromatograms of extracts from the N. benthamiana control, CfTPS1/SsSCS expressed in N. benthamiana or in S. cerevisiae, the in vitro assay, and an authentic standard of (9S,1(0S‐manool (23 a). Total ion current (TIC) and extracted‐ion chromatogram (EIC) levels are not to scale. C) 2D representation of diterpenes detected in N. benthamiana expressing either a single diTPS or combinations of diTPSs from the two classes (y axis). Diterpenes are sorted on the x axis by their Kovats retention indices. Cf=C. forskohlii; Ss=S. sclarea; Mv=M. vulgare; Ep=Euphorbia peplus; Os=O. sativa; Tw=T. wilfordii; Zm=Z. mays. Diterpenes in samples expressing solely class II diTPSs are likely the result of endogenous phosphatase activity and non‐specific rearrangement (Figure S7).6a, 7