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. 2016 Jan 8;55(7):2356–2360. doi: 10.1002/anie.201508642

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© 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Application of iBody 4, which targets His‐tagged proteins. a) Comparison of iBody 4 and anti‐polyhistidine antibody sensitivity for the visualization of purified Ddi1‐His by western blot. b) Comparison of iBody 4 and the anti‐polyhistidine antibody for the visualization of Ddi1‐His in a cell lysate by western blot. c) Affinity isolation of Ddi1‐His by using iBody 4. iBody 5 (which does not possess the tris‐NTA ligand), and blank resin (NC) were used as negative controls. MWM=molecular‐weight marker. d) Binding of Ddi1‐His to immobilized iBody 4 as analyzed by SPR.