Figure 5.

Assessment of the phenotype and polyfunctionality of PRRSV-1 NSP5-specific CD8 T cells. Previously cryopreserved PBMC from identified T cell responder pigs on day 30 postinfection were stimulated with synthetic peptide pools representing M or NSP5 proteins or left unstimulated. The expression of CD44, CD62L, CD25, CD27, surface CD107a, and TNF-α by IFN-γ⁺ CD4 T cells in response to M peptides and CD8 T cells in response to NSP5 peptides were assessed by flow cytometry as shown by representative dot plots. The mean % of unstimulated-corrected data from duplicate cultures are presented for individual animals and error bars show the SEM.