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. 2015 Aug 25;137(35):11303–11311. doi: 10.1021/jacs.5b04366

Figure 6.

Figure 6

Selectivity of P1 in E. coli or HEK293T cell lysate lacking or overexpressing RA. Lysates (3 mg/mL) obtained by sonication were incubated with P1 (10 μM) for 10 min at 25 °C. The samples were fractionated on an SDS-PAGE gel and visualized by either a Bio-Rad Gel Doc Imager employing UV illumination to see the conjugate fluorescence signal or bright field light to observe the Coomassie staining. No significant off-target bands were observed in lysates of cells lacking or expressing RA. FL = RA-P1 conjugate-associated fluorescence, CB = Coomassie blue.