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. 2015 Aug 25;137(35):11303–11311. doi: 10.1021/jacs.5b04366

Figure 8.

Figure 8

RAm1 is a sensor of proteostasis network capacity inside living E. coli cells using solubility as an indicator. After the application of a thermal stress that leads to proteome misfolding and aggregation, RAm1 partitioned into an insoluble state in living E. coli cells after 10 min of a heat stress at 45 °C, as revealed by centrifugation followed by an SDS-PAGE gel visualized by immunoblotting. S = soluble fraction, I = insoluble fraction, T = total protein. Trigger factor was used as a loading control.