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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Biomol Screen. 2015 Jun 4;20(9):1124–1131. doi: 10.1177/1087057115589590

Figure 5. Summary of high throughput screening assay performance.

Figure 5

A. Comparison of the percent inhibition for compounds in two distinct runs of the assay. Note that linearity indicates a highly consistent assay. B. Graph showing the percent inhibition (as an average of the two runs) for the compounds screened. C. Hits identified on the FITC screen were subsequently assayed using a TAMRA labeled HCN peptide to rule out compounds that interact with FITC. D. NUCC-5953 was identified as an inhibitor in both the FITC screen and follow up TAMRA screen. Subsequent dose response testing using the TAMRA labeled HCN probe is shown. E. Structure for compound NUCC-5953 is shown. F. Table with properties of the screening library.