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. Author manuscript; available in PMC: 2016 Feb 16.
Published in final edited form as: Nat Commun. 2015 May 12;6:7079. doi: 10.1038/ncomms8079

Figure 5. Nutritional regulation of gene expression.

Figure 5

IR floxed, LIRKO and LIRFKO mice were fasted overnight for 18 h and then refed or allowed to continue fasting until they were killed 6 h later. Cervical blood was collected for analysis of serum levels of glucose and insulin (Fig. 2) and for levels of (a) corticosterone (N = 8, 4 and 6 fasting, and 4, 5 and 4 refed IR floxed, LIRKO and LIRFKO mice, respectively), and (b) free fatty acids (N = 8, 6 and 6 fasting, and 5, 6 and 6 refed IR floxed, LIRKO and LIRFKO mice, respectively). (c–f) mRNA levels for (c) glucokinase, (d) glucose-6 phosphatase, (e) PEPCK and (f) PDK4 were measured by quantitative PCR in fasting and refed IR floxed (N = 5 fasting, 6 refed), LIRKO (N = 7 fasting, 4 refed) and LIRKO (N = 6 fasting, 5 refed) mice. Disruption of FoxO1 restores the ability of refeeding to regulate the expression of glucokinase, G6Pase and PEPCK in the liver despite the absence of hepatic IR in LIRFKO mice. Results are presented as the mean and s.e.m. *P<0.5 by one way analysis of variance and Fisher’s posthoc test.