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. Author manuscript; available in PMC: 2016 Feb 16.

Published in final edited form as: Methods Enzymol. 2015 Mar 24;557:363–392. doi: 10.1016/bs.mie.2014.12.018

Fig. 1 — Cartoon representation cross-section of a membrane protein solubilized in (a) lipid bilayer of the host cell and (b) detergent introduced during the purification process. Upon removal of the protein from the native lipid (blue) environment, the protein-detergent complex consists of a uniform disordered ring of detergent monomers (red) making hydrophobic contacts with the surface of the protein (green).

Fig. 1 — Cartoon representation cross-section of a membrane protein solubilized in (a) lipid bilayer of the host cell and (b) detergent introduced during the purification process. Upon removal of the protein from the native lipid (blue) environment, the protein-detergent complex consists of a uniform disordered ring of detergent monomers (red) making hydrophobic contacts with the surface of the protein (green).