Table 3. Determining the limit of variant frequency detection.

Variant frequency (%)			Wild type		Mutant
Test sample	NGS result	Depth	Forward reads	Reverse reads	Forward reads	Reverse reads
0.5	1.1	459 772	140 068	310 841	1665	3441
1	1.9	504 779	149 572	340 835	2954	6680
2	2.6	463 811	124 329	323 702	3158	8805
10	10.7	399 956	98 220	255 492	11 222	31 623
20	19.4	440 254	350 826	118 714	26 670	58 759