Using mRNA expression levels of the NAWM and miRNA predication analysis, the potential targets of miR-191 were identified. (A) A correlation analysis was performed between the mRNA expression of WSB1 in the mRNA microarray and miR-191. (B) Two additional mRNA, SOX4 and FZD4, had an inverse relationship with miR-191 expression. Independent real-time PCR analysis of SOX4 (C), FZD5 (D) and BDNF (E) mRNA illustrated an inverse correlation with miR-191, as well as increased levels of these transcripts in NAWM of MS patient brains compared to control.