Inhibition of AR prevents the expression of inflammatory and preneoplastic markers in AOM-treated db/db mice. Colon sections (n = 6) were stained with antibodies against COX-2, iNOS, AR, cyclin D1, PCNA and phospho-NF-κB. Immunoreactivity of the antibody was assessed by quantifying the dark brown stain in the cells, whereas the non-reactive areas displayed only the background color. Photomicrographs of the stained sections were acquired using an EPI-800 microscope (bright-field) connected to a Nikon camera (×400 magnification). Con, control; AOM, azoxymethane; AOM + Fid, azoxymethane + fidarestat. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)