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. Author manuscript; available in PMC: 2017 Feb 4.
Published in final edited form as: Adv Healthc Mater. 2015 Dec 7;5(3):305–310. doi: 10.1002/adhm.201500778

Figure 3.

Figure 3

Release profiles of siRNA into DMEM-HG from 15% (w/w) photolabile hydrogels exposed to no UV and UV light at (A) an intensity of 10 mW/cm2 for 20 (“UV 10-20”) and 60 min (“UV 10-60”) or (B) different intensities of 2, 5 and 10 mW/cm2 for 20 min (“UV 2-20”, “UV 5-20” and “UV 10-20”, respectively). (C) Concentration of siRNA in the releasates. (D) % GFP expression of deGFP-expressing HeLa cells after 2 days of culture with the same concentration (0.5 mL 0.075 μM siRNA) of fresh siGFP and released siRNA collected at day 4 of the release experiment. G1) Control; G2) Gel only, UV 10-20; G3) siLuc, Gel UV 10-20; G4) fresh siGFP; G5) siGFP, Gel No UV; G6) siGFP, Gel UV 2-20; G7) siGFP, Gel UV 5-20; G8) siGFP, Gel UV 10-20; G9) siGFP, Gel UV 10-60. The released siGFP from photolabile hydrogels treated with different UV doses (G5-G9) silenced GFP expression at a similar degree compared to fresh siGFP. Percentage GFP expression of deGFP-expressing HeLa cells after 2 days of culture with the same volume (0.5 mL) of releasates from hydrogels exposed to different UV (E) intensities or (F) exposure durations collected at different time points. # p<0.01 compared to “Control” (G1), * p<0.05.