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. 2016 Feb 16;11(2):e0148774. doi: 10.1371/journal.pone.0148774

Fig 1. Ectopic expression of ZEB1 promotes MDA-MB-231-mediated angiogenesis in vitro.

Fig 1

(A) MDA-MB-231 cells were stably transfected with the human ZEB1 expression plasmid (ZEB1/231) or shRNA plasmid targeting ZEB1 (shZEB1/231). Expression of ZEB1 protein was determined by Western blotting. Actin was used to normalize ZEB1 levels. (B) HUVECs cultured in ZEB1/231- or shZEB1/231-derived conditioned medium were subjected to a tube formation assay and photographed. (C) Quantification of tube formation was expressed as length of capillary tubes formed per mm2. **P < 0.01 vs respective control in Student’s t-test.