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. 2016 Feb 16;11(2):e0148774. doi: 10.1371/journal.pone.0148774

Fig 4. ZEB1 upregulates VEGFA expression by differentially regulating PI3K and p38 pathways.

Fig 4

MDA-MB-231 cells were transiently transfected with the human ZEB1 expression plasmid or empty vector control, followed by treatment with PI-103 (10 μM) or SB203580 (10 μM). At the indicated time points, upregulation of VEGFA expression were verified by qPCR (A and B), Western blotting (C and D) and ELISA (E and F) in ZEB1-expressing versus control cells. GAPDH and actin were used to normalize VEGFA levels. *P < 0.05, **P < 0.01 vs respective control in Student’s t-test.