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. 2016 Feb 16;11(2):e0149023. doi: 10.1371/journal.pone.0149023

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© 2016 Zou et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Growing cells from fragments (left) and MMC-treated hUC-MSCs feeder (right) show property of fibroblast-like cells with a spindle-shaped morphology. Scale bar: 200 μm. (B) The doubling time of different hUC-MSC lines. Each column represented the mean ±SD. (C) Flow cytometry results of rapidly dividing hUC-MSCs show they are positive for MSCs-specific markers (CD73, CD90 and CD105) but negative for CD34, CD45 and HLA-DR. (D) Alizarin Red S staining of osteogenic cells differentiated from hUC-MSCs (left). Scale bar: 100 μm. Oil Red O staining of adipogenic cells differentiated from hUC-MSCs (right). Scale bar: 20 μm.