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. 2016 Feb 16;11(2):e0149023. doi: 10.1371/journal.pone.0149023

Fig 5. Expression of pluripotent markers and bisulphite sequencing analysis in hiPSCs on hUC-MSCs feeder.

Fig 5

Immunocytochemistry staining showed expression of a panel of pluripotent markers including OCT4 (A), SOX2 (B), NANOG (C), SSEA4 (D) and TRA-1-60 (E). Scale bar: 100 μm. (F) Bisulphite sequencing analysis of the OCT4 and NANOG promoters in hiPSCs cocultured on hUC-MSC feeders and parental fibroblast line. White circles represented demethylated CpG dinucleotides; black circles represented methylated CpG dinucleotides. Cross represented sequencing was not achieved. The cell line is indicated to the left of each cluster.