Table 2. Differentially regulated lipids in H2O2-treated young oocytes.
Fold change ≥ 1.5 (p < 0.05).
| Upregulated lipids in H2O2-treated oocytes (26) | Downregulated lipids in H2O2-treated oocytes (35) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Assignment | Precursor Ion | Product Ion | p-value | Ratio* | Assignment | Precursor Ion | Product Ion | p-value | Ratio* |
| DG(36:0) | 642.6 | 607.6 | 0.00 | 41.73 | PI(36:0)** | 881.6 | 607.3 | 0.03 | 0.12 |
| DG(34:0) | 614.5 | 579.5 | 0.00 | 25.51 | PI(34:1)** | 851.6 | 577.3 | 0.00 | 0.13 |
| DG(32:0) | 586.5 | 551.5 | 0.00 | 17.29 | LPI(18:0)** | 615.2 | 341.1 | 0.01 | 0.13 |
| DG(30:0) | 558.5 | 523.5 | 0.01 | 3.77 | PI(34:0)** | 853.6 | 579.3 | 0.02 | 0.13 |
| PC(42:6) | 862.5 | 183.9 | 0.01 | 3.15 | PI(32:0)** | 825.6 | 551.3 | 0.00 | 0.14 |
| DG(36:7) | 628.4 | 593.4 | 0.05 | 2.28 | PI(34:2)** | 849.6 | 575.3 | 0.00 | 0.15 |
| PC(36:0) | 790.5 | 183.9 | 0.02 | 2.06 | PI(36:2)** | 877.6 | 603.3 | 0.02 | 0.16 |
| PC(38:3) | 812.5 | 183.9 | 0.01 | 2.02 | DG(32:1) | 584.5 | 549.5 | 0.03 | 0.22 |
| PC(38:6) | 806.5 | 183.9 | 0.01 | 1.91 | PE(32:1) | 690.4 | 549.3 | 0.02 | 0.24 |
| PC(36:2) | 786.5 | 183.9 | 0.00 | 1.88 | LPS(18:0)** | 554.3 | 341.2 | 0.02 | 0.25 |
| PC(38:2) | 814.5 | 183.9 | 0.05 | 1.85 | SM(d18:1–18:1) | 729.3 | 183.9 | 0.01 | 0.29 |
| LPA(18:1)** | 465.3 | 339.2 | 0.00 | 1.80 | PA(30:1)** | 647.5 | 521.3 | 0.01 | 0.36 |
| PC(34:1) | 760.5 | 183.9 | 0.00 | 1.78 | PA(32:3)** | 671.5 | 545.3 | 0.00 | 0.37 |
| PE(36:4) | 740.4 | 599.3 | 0.01 | 1.76 | PS(36:1)** | 818.6 | 605.3 | 0.00 | 0.37 |
| PC(34:2) | 758.5 | 183.9 | 0.03 | 1.71 | PA(28:1)** | 619.5 | 493.3 | 0.03 | 0.37 |
| PC(36:1) | 788.5 | 183.9 | 0.04 | 1.70 | LPS(22:6)** | 598.3 | 385.2 | 0.00 | 0.38 |
| PE(36:2) | 744.4 | 603.3 | 0.00 | 1.68 | SM(d18:1–22:0) | 787.3 | 183.9 | 0.03 | 0.40 |
| PC(38:5) | 808.5 | 183.9 | 0.04 | 1.68 | PA(28:4)** | 613.5 | 487.3 | 0.00 | 0.40 |
| PC(36:5) | 780.5 | 183.9 | 0.01 | 1.64 | PA(30:0)** | 649.5 | 523.3 | 0.00 | 0.41 |
| PC(34:0) | 762.5 | 183.9 | 0.02 | 1.62 | PA(30:3)** | 643.5 | 517.3 | 0.03 | 0.43 |
| PC(32:0) | 734.5 | 183.9 | 0.02 | 1.61 | PA(28:5)** | 611.5 | 485.3 | 0.02 | 0.43 |
| PE(36:1) | 746.4 | 605.3 | 0.04 | 1.61 | PS(36:2)** | 816.6 | 603.3 | 0.03 | 0.48 |
| PC(36:4) | 782.5 | 183.9 | 0.02 | 1.58 | LPS(18:1)** | 552.3 | 339.2 | 0.01 | 0.53 |
| PE(38:4) | 768.4 | 627.3 | 0.03 | 1.55 | SM(d18:1–18:0) | 731.3 | 183.9 | 0.04 | 0.53 |
| PG(36:3) | 790.4 | 601.3 | 0.05 | 1.53 | Cer1P(d18:1–16:0)** | 646.5 | 264.1 | 0.01 | 0.56 |
| PE(36:3) | 742.4 | 601.3 | 0.03 | 1.50 | PS(28:0)** | 708.6 | 495.3 | 0.01 | 0.59 |
| LPA(18:3)** | 461.3 | 335.2 | 0.00 | 0.60 | |||||
| ChE(18:1) | 668.6 | 369.2 | 0.00 | 0.60 | |||||
| SO(d14:1) | 244.3 | 208.1 | 0.00 | 0.61 | |||||
| Cer1P(d18:1–16:1)** | 644.5 | 264.1 | 0.01 | 0.61 | |||||
| SM(d18:1–14:0) | 675.3 | 183.9 | 0.01 | 0.63 | |||||
| LPI(14:0)** | 559.2 | 285.1 | 0.00 | 0.63 | |||||
| ChE(22:6) | 714.6 | 369.2 | 0.00 | 0.64 | |||||
| LPC(20:4) | 544.1 | 183.9 | 0.01 | 0.67 | |||||
| SM(d18:1–24:1) | 813.3 | 183.9 | 0.01 | 0.67 | |||||
* Ratio, aged oocytes/young oocytes.
** Acidic lipids analyzed after TMSD derivatization.