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. 2016 Feb 1;32(1):8–15. doi: 10.5423/PPJ.OA.08.2015.0157

Fig. 1.

Fig. 1

Mycelial growth inhibitory effect of L. foenum-graecum Herba extract on M. oryzae. (A) No treatment. L. foenum-graecum Herba extract was applied to (B), (C), (D), (E), and (F) at concentrations of 10 μg/ml, 20 μg/ml, 50 μg/ml, 100 μg/ml and 200 μg/ml, respectively. Combinational inhibitory effect tests on the mycelial growth of M. oryzae. (G) No treatment, (H) 63.06 μg/ml (IC50) of blasticidin S, (I) 25 μg/ml of tetrandrine, (J) combinational treatment of 63.06 μg/ml (IC50) of blasticidin S with 25 μg/ml of tetrandrine, (K) 39.28 μg/ml (IC50) of L. foenum-graecum Herba extract, (L) combinational treatment of 39.28 μg/ml (IC50) of L. foenum-graecum Herba with 63.06 μg/ml (IC50) of blasticidin S, and (M) combinational treatment of 39.28 μg/ml (IC50) of L. foenum-graecum Herba with 25 μg/ml (IC50) of tetrandrine. M. oryzae mycelia cut by cork borer and incubated at 28 ºC on PDA solid media containing the plant extract for five days.