Author response image 3. Yeast agglutination assays on uropathogentic E. coli clinical isolates.
Representative images of yeast agglutination assay. The assay was performed incubating 10 µl of previously identified bacteria isolates (haemagglutination-positive isolates #9, 12, 10), grown in static condition (final concentration 2x109 cells/ml), with 10 µl of S. cerevisiae yeast strain W303 Mata (final concentration of 109 cells/ml). The appearance of white aggregates (arrows) indicates agglutination. UTI isolates were pre-incubated with D- mannose at a final concentration of 2.5% in order to identify mannose-sensitive agglutination, indicative of type 1-fimbriated phenotype. The assay was carried out in 3 replicates.