Figure 4. Liquid droplet (LD)–LD docking and CIDEA interactions.
(A) A Hela cell expressing CIDEA-(152–217)-v5 showing normal recruitment to LDs, but no LD docking. A Hela cell expressing CIDEA-△(126–155)-v5 showing normal LD–LD docking but inefficient LD enlargement as revealed by the presence of clusters of small and large LDs. Representative images are shown of experiments performed in a minimum of three independent experiments for every construct (n>50 cells). (B) Co-immunoprecipitation (co-IP) assays between CIDEA-HA and different CIDEA-v5 constructs. The observed CIDEA–CIDEA interaction was driven by the C-term domain and required the presence of the 126–155 aa sequence. (C, D) Co-IP assays showing CIDEA interactions with CIDEB, CIDEC, and PLIN5. Each co-IP assay was performed at least in triplicate, producing similar results in each experiment.