Figure 6. LPA/LPA2-mediated signaling promotes T-cell migration on a 2D surface.
CD4+ T cells from WT or Lpar2-/- mice were left untreated or were treated with LPA (1 μM) and immediately loaded on one side of an EZ-Taxiscan chamber, and medium containing CCL21 (100 ng) was applied on the other side. (A) Cell migration on a surface coated with ICAM-1-Fc was monitored at 1-min intervals. (B) Cells that migrated toward the CCL21-containing contra-wells were counted, and the (C) average cell velocity and (D) turning angle were calculated. Bars represent the median. Differences between groups were evaluated by Student’s t-test. *P < 0.05, **P < 0.005.