Figure 10. JCasp does not compete for the interaction of amyloid precursor protein (APP) like protein-2 (APLP2) intracellular domain (ALID2) with Syt2, Syp, and Vamp2.
Brain lysates were incubated for 30 min with 10 μM of either JCasp or ScJCasp. After incubation, the lysates were affinity purified on StrepTactin columns bound to the indicated St-peptides. Bound proteins were analyzed by Western blot. JCasp does not reduce binding of Syp, Vamp2, and Syt2 to St-ALID2. The graphs at the bottom of the figure show quantitative analysis of the Western blot data.