Skip to main content
. 2015 Nov 9;4:e09743. doi: 10.7554/eLife.09743

Figure 9. Amyloid precursor protein (APP) is required for the inhibitory effect of Pen1-JCasp on synaptic transmission.

Figure 9.

(A) Analysis of slopes of each input/output (I/O) recording by one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test showed no significant differences among the groups. However, uncorrected Fisher’s LSD test shows that Pen1-JCasp reduces basal synaptic transmission in wild-type (WT) hippocampal slices as compared to WT slices treated with Pen1-ScJCasp. Pen1-JCasp increases paired-pulse facilitation (PPF) (B) and frequency facilitation (FF) (C) in WT but not App-KO excitatory Schaffer collateral (SC) synapses. Representative traces are shown on the right of summary plots. The number of recordings and of mice analyzed for each group are shown in (A). All data represent means ± SEM. Statistical assessment by two-way repeated measures (RM) ANOVA shows significant differences in PPF and FF between WT mice treated with Pen1-JCasp and the other three experimental groups (*, vs. WT+Pen1-SCJCasp; *, vs. App-KO+Pen1-SCJCasp; *, vs. App-KO+Pen1-JCasp; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). The complete statistical analyses are shown in the attached Excel file.

DOI: http://dx.doi.org/10.7554/eLife.09743.025

Figure 9—source data 1. Source data for statistical analysis of input/output curves in Figure 9A.
DOI: http://dx.doi.org/10.7554/eLife.09743.026
elife-09743-fig9-data1.xlsx (53.1KB, xlsx)
DOI: 10.7554/eLife.09743.026
Figure 9—source data 2. Source data for statistical analysis of paired-pulse facilitation in Figure 9B.
DOI: http://dx.doi.org/10.7554/eLife.09743.027
elife-09743-fig9-data2.xlsx (3.6MB, xlsx)
DOI: 10.7554/eLife.09743.027
Figure 9—source data 3. Source data for statistical analysis of frequency facilitation at 20 Hz in Figure 9C.
DOI: http://dx.doi.org/10.7554/eLife.09743.028
elife-09743-fig9-data3.xlsx (3.6MB, xlsx)
DOI: 10.7554/eLife.09743.028