Figure 2. PCH1 regulates the photoperiodic response of hypocotyl elongation in the evening.
(A) Hypocotyl lengths of 4-day-old WT, pch1, PCH1ox3, elf3-2, elf4-2 and phyB-9 seedlings grown under long day, 12L:12D and short day conditions. Mean ± 95% confidence interval (CI) (n=20). (B) pch1 grows faster than WT during night. Time-lapse images were taken every hour for each seedling of WT, pch1, PCH1ox3 and PCH1p::PCH1 grown under short day conditions. Growth rate was calculated as the hypocotyl increase per hour and plotted against time. Solid lines are the regression analyses of data. Mean ± SEM (n ≥ 14). Grey shading indicates dark period. Also see Figure 2—source data 1 and 2.
DOI: http://dx.doi.org/10.7554/eLife.13292.009
Figure 2—source data 1. Raw measurements of hypocotyl lengths for Figure 2A.
Hypocotyl lengths (mm) of 4-day-old WT, pch1, PCH1ox3, elf3-2, elf4-2 and phyB-9 seedlings grown under long day, 12L:12D and short day conditions, with n = 20.
elife-13292-fig2-data1.xlsx (14.3KB, xlsx)
DOI: 10.7554/eLife.13292.010
Figure 2—source data 2. ANOVA analyses and Bonferroni's multiple comparison tests for Figure 2A.
2-way ANOVA analyses were carried out using GraphPad Prism (version 6.0, Graphpad.com, La Jolla, California), with Bonferroni's multiple comparisons test results.
elife-13292-fig2-data2.xlsx (27.6KB, xlsx)
DOI: 10.7554/eLife.13292.011
Figure 2—figure supplement 1. PCH1 levels regulate hypocotyl length under short day conditions.
Hypocotyl lengths of short-day-grown, 4-day-old plants of WT, pch1, two independent overexpression lines (PCH1ox3 and 4, in WT) and two complementation lines (PCH1p::PCH1-7 and -8, in pch1) were measured. Inset photo of each genotype with scale bar = 5 mm. Mean ± 95% CI (n=20). (****p<0.0001, ns = not significant, each compared to WT).