Figure 6. pch1 exhibits defects in red light responsive hypocotyl growth and expression of downstream transcription factors.

(A) Hypocotyl lengths of 4-day-old WT, pch1, PCH1ox3, PCH1p::PCH1 and phyB-9 seedlings grown under either dark or constant red light of various intensities (25, 40 and 100 µmol·m^-2·s^-1). Mean ± 95% CI (n = 20). Hypocotyl lengths of light-grown seedlings were normalized to dark-grown (etiolated) seedlings, and were plotted against light intensities to generate the responsive curve. Etiolated hypocotyl lengths (mean ± SD) of WT, pch1, PCH1ox3, PCH1p::PCH1 and phyB-9 are 9.02 ± 0.90, 8.47 ± 0.66, 8.04 ± 0.70, 8.71 ± 0.71 and 7.63 ± 0.79, respectively. (B) qPCR of HFR1, ATHB-2 using time-course cDNA samples of short-day grown, 4-day-old WT, pch1 and PCH1ox3 seedlings. Expression was normalized to IPP2 and APA1. Mean ± SD (n=3 biological reps). Grey shading indicates dark period.

DOI: http://dx.doi.org/10.7554/eLife.13292.022

Figure 6—figure supplement 1. pch1 does not affect far-red or blue light mediated hypocotyl elongation.

(A) Hypocotyl measurements of 4-day-old WT, pch1, PCH1ox3 and phyA-211 seedlings grown under either dark or constant far-red light conditions (FRc, 25 µmol·m^-2·s^-1). Mean ± 95% CI (n = 20). (B) Hypocotyl measurements of 4-day-old WT (Col), pch1, PCH1ox3, Wassilewskija (WS) and cry1 cry2 (in WS) seedlings under either dark or constant blue light conditions (Bc, 20 µmol·m^-2·s^-1). Mean ± 95% CI (n = 20).

DOI: http://dx.doi.org/10.7554/eLife.13292.023