Fig. 4. p38 MAPK modulates Slack K_Na current in Slack stable HEK cells.

Representative traces of whole-cell recordings (Top) and current voltage relationship of Slack current (Bottom) from (a) Slack Stable HEK cells treated with vehicle (0.01% DMSO) or anisomycin (10 μg/ml) for 90 minutes at 37°C, (b) Serum starved slack stable HEK cells treated with vehicle (0.01% DMSO) or anisomycin (10 μg/ml) for 90 minutes at 37°C, (c) Slack Stable HEK cells treated with vehicle (0.01% DMSO) or p38 Inhibitor (10 μM) for 90 minutes at 37°C. Data is means ± SEM via unpaired t-test (n=7–10 per group; *p<0.05 between vehicle and p38 Inhibitor/anisomycin groups). (d) Representative traces of whole-cell recordings (Top) and current voltage relationship of Slack current (Bottom) from Slack Stable HEK cells treated with vehicle or p38 Inhibitor alone or p38 Inhibitor following anisomycin treatment. Data is expressed as mean +/− SEM (n=7–10 for all groups). Statistics performed using One Way ANOVA followed by multiple comparisons using Tukey’s method (n=7–10 per group; *p<0.05 between vehicle and p38 Inhibitor/anisomycin groups, ^#p<0.05 between vehicle and Inhibitor + anisomycin groups.) Holding potential was −70 mV, and currents were elicited with voltage steps from −120 mV to +120 mV in 20 mV increments.