Neuronal cultures were supplemented at day 1 with either 3 μM EPA, or 4 μM oleic (OLA), palmitic (PAM), or arachidonic (ARA) acid, or vehicle (−) and treated with PQ at day 3. Bars depict the percentage of PI-labeled cells (a) and of photoreceptors with fragmented or pyknotic (apoptotic) nuclei (b). Three experiments with three dishes for each condition were analyzed and 10 fields per dish were counted blindly in all experiments. *p≤0.05, statistically significant differences determined by a one-way ANOVA test followed by a Tukey’s test.