Figure 3.
(A) Selected cryo-TEM micrographs showing a general structure of L35-PR+:siRNA and L81-PR+:siRNA at ρeff = 3. The insets showing a FFT profile from a particular area of the polyplexes. (B) SAXS diffractograms of PR+:siRNA complexes at effective charge ratios of 2 and 3. (C) Scheme representing the multilamellar arrangement constituted by a series of PR+ monolayers with siRNA compacted and sandwiched between them. The interlamellar spacing (dm) was found to be ~4.8 and thickness of siRNA (dw) to be ~ 3.2 nm (figure drawn to arbitrary scale)