Table 1.
Proteins forming covalent cross-links to chromosomal DNA following exposure to PM.
| Swiss-Prot ID | Identified Protein | % Coverage | No. of Unique Peptides | Total Spectra | Primary Biological Function | Protein MW (Da) | |
|---|---|---|---|---|---|---|---|
| 1 | P53999 | Activated RNA polymerase II transcriptional coactivator p15 | 37 | 4 | 4 | Transcriptional Regulation | 14395.9 |
| 2 | O15347 | High mobility group protein B3 | 19 | 3 | 3 | 22980.7 | |
| 3 | P39687 | Acidic leucine-rich nuclear phosphoprotein 32 family member A | 21 | 5 | 7 | 28586.1 | |
| 4 | Q99623 | Prohibitin-2 | 10 | 2 | 2 | 33297.6 | |
| 5 | P16989 | DNA-binding protein A | 8 | 2 | 2 | 40089.4 | |
| 6 | Q14320 | Protein FAM50A | 8 | 2 | 2 | 40242.8 | |
| 7 | Q5BKZ1 | Zinc finger protein 326 | 11 | 4 | 4 | 65653.5 | |
| 8 | P11142 | Heat shock cognate 71 kDa protein | 8 | 3 | 3 | 70899.8 | |
| 9 | Q8WVC0 | RNA polymerase-associated protein LEO1 | 4 | 2 | 2 | 75405.3 | |
| 10 | Q9Y3T9 | Nucleolar complex protein 2 | 3 | 2 | 2 | 84907.1 | |
| 11 | P43243 | Matrin-3 | 13 | 10 | 11 | 94626.7 | |
| 12 | P55884 | Eukaryotic translation initiation factor 3 subunit B | 22 | 15 | 18 | 92482.8 | |
| 13 | Q8TDD1 | ATP-dependent RNA helicase DDX54 | 2 | 2 | 2 | 98597.9 | |
| 14 | Q9BXP5 | Serrate RNA effector molecule homolog | 3 | 3 | 3 | 100669.7 | |
| 15 | Q9Y5B9 | FACT complex subunit SPT16 | 16 | 15 | 17 | 119917.4 | |
| 16 | Q03701 | CCAAT/enhancer binding protein zeta | 2 | 2 | 2 | 120992.2 | |
| 17 | Q9BQG0 | Myb-binding Protein 1A | 2 | 2 | 2 | 148858.3 | |
| 18 | P51532 | Transcription activator BRG1 | 2 | 3 | 3 | 184649.6 | |
| 19 | O60216 | Double-strand-break repair protein rad21 homolog | 6 | 3 | 3 | DNA Repair | 71691.6 |
| 20 | P12956 | X-ray repair cross-complementing protein 5 | 22 | 10 | 12 | 69846.4 | |
| 21 | P84103 | Splicing factor, arginine/serine-rich 3 | 30 | 5 | 5 | RNA Splicing/Processing | 19330.0 |
| 22 | Q07955 | Splicing factor, arginine/serine-rich 1 | 12 | 3 | 3 | 27745.1 | |
| 23 | Q32P51 | Heterogeneous nuclear ribonucleoprotein A1-like 2 | 15 | 4 | 5 | 34225.4 | |
| 24 | P22626 | Heterogeneous nuclear ribonucleoprotein A2/B1 | 10 | 3 | 3 | 37430.3 | |
| 25 | P08621 | U1 small nuclear ribonucleoprotein 70 kDa | 5 | 2 | 2 | 51558.4 | |
| 26 | O00566 | U3 small nucleolar ribonucleoprotein protein MPP10 | 7 | 3 | 3 | 78866.8 | |
| 27 | Q15029 | 116 kDa U5 small nuclear ribonucleoprotein component | 4 | 3 | 3 | 109438.1 | |
| 28 | Q15393 | Splicing factor 3B subunit 3 | 2 | 2 | 2 | 135579.4 | |
| 29 | O75643 | U5 small nuclear ribonucleoprotein 200 kDa helicase | 2 | 4 | 4 | 244513.8 | |
| 30 | P51665 | 26S proteasome non-ATPase regulatory subunit 7 | 18 | 3 | 3 | Apoptosis | 37025.6 |
| 31 | P04083 | 26S proteasome non-ATPase regulatory subunit 3 | 19 | 9 | 9 | 60979.6 | |
| 32 | Q9NY61 | Protein AATF | 22 | 7 | 8 | 63135.0 | |
| 33 | P25205 | DNA replication licensing factor MCM3 | 6 | 4 | 4 | DNA Replication | 90982.6 |
| 34 | Q14566 | DNA replication licensing factor MCM6 | 3 | 2 | 2 | 92890.2 | |
| 35 | P33778 | Histone H2B type 1-B | 20 | 3 | 14 | Chromatin Organization | 13950.8 |
| 36 | Q92522 | Histone H1× | 10 | 2 | 2 | 22487.9 | |
| 37 | Q9P0M6 | Core histone macro-H2A.2 | 7 | 2 | 3 | 40060.1 | |
| 38 | Q969G3 | SWI/SNF-related matrix associated actin dependent regulator of chromatin subfamily E member 1 | 13 | 4 | 5 | 46649.7 | |
| 39 | Q14839 | Chromodomain-helicase-DNA-binding protein 4 | 4 | 6 | 6 | 217994.6 | |
| 40 | Q12873 | Chromodomain-helicase-DNA-binding protein 3 | 1 | 2 | 2 | 226597.2 | |
| 41 | P63173 | 60S ribosomal protein L38 | 29 | 2 | 4 | Translation | 8218.5 |
| 42 | P62979 | 40S ribosomal protein S27a | 40 | 2 | 3 | 9418.4 | |
| 43 | Q6NW1 | Putative 60S ribosomal protein L13a-like MGC87657 | 29 | 3 | 3 | 12135.0 | |
| 44 | P83881 | 60S ribosomal protein L36a | 30 | 4 | 4 | 12441.1 | |
| 45 | Q5JNZ5 | Putative 40S ribosomal protein S26-like 1 | 27 | 3 | 10 | 13002.2 | |
| 46 | P60866 | 40S ribosomal protein S20 | 19 | 2 | 3 | 13373.0 | |
| 47 | P62899 | 60S ribosomal protein L31 | 30 | 4 | 11 | 14463.2 | |
| 48 | P35268 | 60S ribosomal protein L22 | 19 | 2 | 3 | 14787.3 | |
| 49 | P62244 | 40S ribosomal protein S15a | 31 | 4 | 4 | 14840.0 | |
| 50 | P62829 | 60S ribosomal protein L23 | 32 | 3 | 4 | 14865.9 | |
| 51 | P62847 | 40S ribosomal protein S24 | 29 | 4 | 9 | 15423.8 | |
| 52 | P08708 | 40S ribosomal protein S17 | 16 | 2 | 3 | 15550.5 | |
| 53 | P62266 | 40S ribosomal protein S23 | 15 | 2 | 3 | 15807.7 | |
| 54 | P62263 | 40S ribosomal protein S14 | 34 | 4 | 4 | 16272.9 | |
| 55 | P62249 | 40S ribosomal protein S16 | 28 | 4 | 6 | 16445.9 | |
| 56 | P46776 | 60S ribosomal protein L27a | 20 | 3 | 3 | 16561.4 | |
| 57 | P62841 | 40S ribosomal protein S15 | 40 | 3 | 6 | 17040.8 | |
| 58 | P62277 | 40S ribosomal protein S13 | 36 | 6 | 7 | 17223.3 | |
| 59 | P30050 | 60S ribosomal protein L12 | 24 | 3 | 4 | 17819.1 | |
| 60 | P62280 | 40S ribosomal protein S11 | 35 | 5 | 5 | 18431.3 | |
| 61 | P46778 | 60S ribosomal protein L21 | 21 | 3 | 8 | 18565.0 | |
| 62 | P46783 | 40S ribosomal protein S10 | 15 | 2 | 4 | 18898.3 | |
| 63 | Q9NQ39 | Putative 40S ribosomal protein S10-like | 13 | 2 | 3 | 20120.6 | |
| 64 | Q02543 | 60S ribosomal protein L18a | 21 | 3 | 4 | 20762.6 | |
| 65 | T1W3K1 | 60S ribosomal protein L17 | 41 | 6 | 11 | 21397.4 | |
| 66 | P32969 | 60S ribosomal protein L9 | 18 | 3 | 4 | 21863.7 | |
| 67 | P62081 | 40S ribosomal protein S7 | 47 | 6 | 10 | 22127.5 | |
| 68 | P46781 | 40S ribosomal protein S9 | 45 | 12 | 21 | 22592.5 | |
| 69 | P23396 | 40S ribosomal protein S3 | 27 | 5 | 5 | 26688.6 | |
| 70 | P62753 | 40S ribosomal protein S6 | 18 | 4 | 5 | 28681.7 | |
| 71 | P18124 | 60S ribosomal protein L7 | 38 | 12 | 16 | 29227.7 | |
| 72 | P15880 | 40S ribosomal protein S2 | 24 | 6 | 6 | 31325.2 | |
| 73 | P05198 | Eukaryotic translation initiation factor 2 subunit 1 | 10 | 2 | 2 | 36112.7 | |
| 74 | P20042 | Eukaryotic translation initiation factor 2 subunit 2-like protein | 16 | 5 | 5 | 37788.3 | |
| 75 | P60842 | Eukaryotic initiation factor 4A-I | 22 | 7 | 7 | 46155.3 | |
| 76 | O15371 | Eukaryotic translation initiation factor 3 subunit D | 11 | 5 | 5 | 63973.8 | |
| 77 | P55884 | Eukaryotic translation initiation factor 3 subunit B | 22 | 15 | 18 | 92482.8 | |
| 78 | Q14152 | Eukaryotic translation initiation factor 3 subunit A | 12 | 15 | 18 | 166574.9 | |
| 79 | Q04637 | Eukaryotic translation initiation factor 4 gamma 1 | 9 | 13 | 14 | 175494.2 | |
| 80 | P05109 | Protein S100 A8 | 32 | 3 | 4 | Inflammatory Response | 10835.0 |
| 81 | P06702 | Protein S100 A9 | 45 | 4 | 6 | 13242.3 | |
| 82 | P66905 | Hemoglobin subunit alpha | 22 | 2 | 3 | Oxygen Transport | 15257.6 |
| 83 | Q13765 | Nascent polypeptide-associated complex subunit alpha | 26 | 4 | 5 | Protein Transport | 23383.3 |
| 84 | Q96FZ7 | Charged multivesicular body protein 6 | 10 | 2 | 2 | 23485.3 | |
| 85 | O76021 | Ribosomal L1 domain-containing protein 1 | 11 | 4 | 4 | 54974.7 | |
| 86 | O00203 | AP-3 complex subunit beta-1 | 5 | 4 | 4 | 121324.0 | |
| 87 | Q9P2E9 | Ribosome-binding protein 1 | 14 | 16 | 21 | 152470.6 | |
| 88 | Q08378 | Golgin subfamily A member 3 | 3 | 3 | 3 | 167356.0 | |
| 89 | Q00610 | Clathrin heavy chain 1 | 11 | 15 | 15 | 191618.9 | |
| 90 | Q13268 | Dehydrogenase/reductase SDR family member 2, mitochondrial | 11 | 3 | 3 | Cellular Homeostasis | 27438.4 |
| 91 | Q15006 | Tetratricopeptide repeat protein 35 | 18 | 4 | 4 | 34834.8 | |
| 92 | P00338 | L-lactate dehydrogenase A chain | 13 | 4 | 5 | 36689.2 | |
| 93 | P11201 | 78 kDa glucose-regulated protein | 17 | 8 | 8 | 72334.7 | |
| 94 | P04083 | Annexin A1 | 27 | 6 | 8 | 38715.9 | |
| 95 | Q96A33 | Coiled-coil domain-containing protein 47 | 9 | 3 | 3 | 55874.9 | |
| 96 | P25705 | ATP synthase subunit alpha, mitochondrial | 12 | 6 | 6 | 59752.1 | |
| 97 | Q9NR30 | Nucleolar RNA helicase 2 | 6 | 4 | 4 | 87346.0 | |
| 98 | P36776 | Lon protease homolog, mitochondrial | 6 | 5 | 6 | 106491.3 | |
| 99 | P63104 | 14-3-3 protein zeta/delta | 17 | 3 | 4 | Cellular Signaling | 27745.9 |
| 100 | P31946 | 14-3-3 protein beta/alpha | 9 | 2 | 5 | 28083.1 | |
| 101 | P62258 | 14-3-3 protein epsilon | 12 | 3 | 3 | 29175.0 | |
| 102 | Q9UBS4 | DnaJ homolog subfamily B member 11 | 14 | 3 | 3 | 40514.6 | |
| 103 | Q3TIV5 | Zinc finger CCCH domain-containing protein 15 | 10 | 3 | 3 | 48603.7 | |
| 104 | Q13283 | Ras GTPase-activating protein-binding protein 1 | 9 | 3 | 4 | 52162.8 | |
| 105 | Q1KMD3 | Heterogeneous nuclear ribonucleoprotein U-like protein 2 | 9 | 6 | 7 | 85105.2 | |
| 106 | P05556 | Integrin beta-1 | 6 | 4 | 4 | 88415.1 | |
| 107 | O14786 | Neuropilin-1 | 19 | 12 | 13 | 103136.6 | |
| 108 | P08865 | 40S ribosomal protein SA | 19 | 3 | 3 | Cell Structure/Architecture | 32854.1 |
| 109 | P60709 | Actin, cytoplasmic 1 | 19 | 5 | 6 | 41737.8 | |
| 110 | P68363 | Tubulin alpha-1B chain | 6 | 2 | 5 | 50151.7 | |
| 112 | P55081 | Microfibrillar-associated protein 1 | 17 | 5 | 6 | 51958.7 | |
| 113 | P17661 | Desmin | 4 | 2 | 8 | 53536.6 | |
| 114 | P41219 | Peripherin | 4 | 2 | 10 | 53652.0 | |
| 115 | P08670 | Vimentin | 67 | 30 | 142 | 53652.7 | |
| 116 | Q16352 | Alpha-internexin | 6 | 3 | 4 | 55392.3 | |
| 117 | Q07065 | Cytoskeleton-associated protein 4 | 48 | 23 | 27 | 66022.2 | |
| 118 | Q16643 | Drebrin | 6 | 2 | 2 | 71428.6 | |
| 119 | P23284 | Peptidyl-prolyl cis-trans isomerase B | 21 | 4 | 4 | Protein Folding | 23743.2 |
| 120 | Q9UKD2 | mRNA turnover protein homolog 4 | 16 | 3 | 3 | Ribosome Assembly | 27561.3 |
| 121 | Q99848 | Probable rRNA-processing protein EBP2 | 27 | 6 | 8 | 34852.9 | |
| 122 | Q15050 | Ribosome biogenesis regulatory protein homolog | 24 | 7 | 9 | 41194.4 | |
| 123 | O00541 | Pescadillo homolog | 6 | 4 | 4 | 68004.9 | |
| 124 | Q14692 | Ribosome biogenesis protein BMS1 | 4 | 3 | 3 | 145812.2 | |
| 125 | P54652 | Heat shock-related 70 kDa protein 2 | 5 | 3 | 3 | Cell Cycle | 70023.0 |
| 126 | Q5TAP6 | U3 small nucleolar RNA-associated protein 14 homolog A | 12 | 7 | 7 | 87979.4 | |
| 127 | P46087 | Putative ribosomal RNA methyltransferase NOP2 | 9 | 5 | 5 | 89303.6 | |
| 128 | Q8WTT2 | Nucleolar complex protein 3 homolog | 3 | 2 | 2 | 92552.2 | |
| 129 | A0LTM5 | Cell division protein 11B | 3 | 2 | 2 | 92709.0 | |
| 130 | O75400 | Pre-mRNA-processing factor 40 homolog A | 5 | 5 | 5 | 108807.3 | |
| 131 | Q14980 | Nuclear mitotic apparatus protein 1 | 5 | 9 | 10 | 238259.7 | |
| 132 | Q9BXY0 | Protein MAK16 homolog | 15 | 3 | 4 | Unknown | 35370.0 |
| 133 | Q14257 | Reticulocalbin-2 | 32 | 9 | 12 | 36877.6 | |
| 134 | Q9NZM5 | Glioma tumor suppressor candidate region gene 2 protein | 14 | 6 | 6 | 54390.7 |