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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Cell Physiol. 2015 Nov 4;231(6):1237–1248. doi: 10.1002/jcp.25219

Figure 2. Human synaptic plasticity array mRNA was generated from a mixed culture of neurons and neuronal progenitor cells.

Figure 2

Cells were plated to about 70% confluency in neuronal media for 5 days and treatment of cells was initiated: Control – no treatment, TNF-α – 100ng/mL every four hours for three days. A. TUJ1 staining that confirmed presence of neurons, Nestin staining that confirmed neural progenitor cells, and GFAP that confirmed glial populations. TNF-α upregulates numerous genes involved in regulation of synaptic plasticity. B. Quantitative RTPCR data from human neural stem cells after administering TNF-α show upregulation of EphB2, GRIP, and NF-κB. mRNA expression for each gene was normalized to β-actin control expression.