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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Cell Physiol. 2015 Nov 4;231(6):1237–1248. doi: 10.1002/jcp.25219

Figure 7. NF-κB binding sites 2 and 3 are primarily responsible for EphB2 promoter activation in response to TNF-α.

Figure 7

Human primary neurons were transfected with pGL3-Basic EPHB2 (−840/+83) and site-directed mutagenesis was performed. A 26% increase was observed in wild type in response to TNF-α, but only a 15% and 18% increase was observed in response to TNF-α when sites 2 and 3 are mutated, respectively. Only a 5% increase was observed when both site 2 and 3 were mutated. Luciferase activity (RLU/s) was normalized to protein concentration in each condition and is represented as RLU/s/µg of protein.