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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Cell Physiol. 2015 Nov 4;231(6):1237–1248. doi: 10.1002/jcp.25219

Figure 8. TNFR2 signaling upregulates EPHB2.

Figure 8

Human primary neurons were cultured for 14 days and treated with the following blocking conditions: IgG antibody (2µg/mL), TNFR1 antibody (2µg/mL), and TNFR2 antibody (2µg/mL) for 30 minutes in serum free media. Following blocking of the TNFRs, TNF-α was then added to the experimental group at a concentration of 100ng/mL. Treatment was allowed to persist for 24 hours and repeated 30 minutes prior to harvest, and cells were harvested. EphB2 mRNA levels were normalized to β-actin expression. EphB2 was induced by 77% by TNF-α treatment in the nonspecific IgG control sample. Upon blocking TNFR1, EphB2 was induced by 55% by TNF-α treatment but only 16% when TNFR2 was blocked. These results indicate that TNF-α induces EphB2 primarily through TNFR2.