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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Laryngoscope. 2015 Sep 7;126(3):E110–E117. doi: 10.1002/lary.25581

Table 1.

Primer sequences and products of RT-PCR.

Gene GenBank # Forward primer Reverse primer Size of PCR product (bp)
Collagen 114 NM_000089 5’-AACAAATAAGCCATCACGCCTGCC-3’ 5’-TGAAACAGACTGGGCCAATGTCCA- 3’ 101
Collagen 314 NM_000090 5’-CCATTGCTGGGATTGGAGGTGAAA-3’ 5’-TTCAGGTCTCTGCAGTTTCTAGCGG- 3’ 187
Fibronectin14 NM_002026 5’-ACCTACGGATGACTCGTGCTTTGA-3’ 5’-CAAAGCCTAAGCACTGGCACAACA-3’ 116
α-SMA17 NM_001141945 5’-CCAGCAGATGTGGATCAGCAAACA-3’ 5’-ACGAGTCAGAGCTTTGGCTAGGAA-3’ 179
MMP117 NM_002421 5’-TGCAACTCTGACGTTGATCCCAGA-3’ 5’-ACTGCACATGTGTTCTTGAGCTGC-3’ 122
TIMP17 NM_000362 5’-TGATGCAGCACACACAATTCCC-3’ 5’-AAGCTCTGTTATTCTGGCCTGGGT-3’ 102
CTGF NM_001901.2 5’-GCTAGGATGTGCATTCTCC-3’ 5’-CCGATTCCTGAACAGTGTC-3’ 112
HGF18 NM_000601 5’-GGCCCACTTGTTTGTGAGCAACAT-3’ 5’-TGGTGGGGTGCTTCAGACACACTTA-3’ 84
TGFβ116 NM_000660 5’-TGCTCGCCCTGTACAACAGCA-3’ 5’-CGTTGTGGGTTTCCACCATTAGCA-3’ 126
VEGF18 NM_001025366 5’-ACACATTGTTGGAAGAAGCAGCCC-3’ 5’-AGGAAGGTCAACCACTCACACACA-3’ 179
GAPDH NM_002046.4 5’-TGAAGGTCGGAGTCAACGGATT-3’ 5’-TTGACGGTGCCATGGAATTTGC-3’ 172

α-SMA – Alpha Smooth Actin; MMP1 - Matrix Metallopeptidase; TIMP3- Metallopeptidase Inhibitor; CTGF- Connective Tissue Growth Factor; HGF- Hepatocyte Growth Factor; TGFβ1- Transforming Growth Factor β1; VEGF- Vascular Endothelial Growth Factor; GAPDH- Glyceraldehydes-3-phosphate dehydrogenase.