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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Cancer Res. 2015 Nov 18;14(2):207–215. doi: 10.1158/1541-7786.MCR-15-0321

Figure 2.

Figure 2

(A) Immunoblot of MEK1 and β-ACTIN (loading control) protein levels 5 days after lentiviral infection of control shRNAs (GFP and LacZ) and two independent MAP2K1 shRNAs in NCI-H1437 and A549 cells.

(B) Cell viability beginning 7 days after lentiviral infections. Viability is normalized to mock infected controls.

(C) Immunoblot of MEK1 and β-ACTIN (loading control) protein levels from pooled population of cells 5 days after lentiviral infection of Cas9 with two independent control sgRNAs each targeting either GFP or MAP2K1 in NCI-H1437 and A549 cells.

(D) Cell viability beginning 7 days after Cas9/sgRNA infections. Viability is normalized to control GFP sgRNA-1 for each cell line on each day.