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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Genet Metab. 2015 Nov 11;117(2):210–216. doi: 10.1016/j.ymgme.2015.11.004

Figure 1. Histochemical substrate for detecting PPT1 enzyme activity in situ.

Figure 1

Step (1) is performed by PPT1. (A) Natural substrate for PPT1; (B) Histochemical substrate. When the indole is released by added β-glucosidase, the indole oxidizes and polymerizes in the presence of added reagents and develops a blue color.