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. 2016 Feb 17;21(2):025007. doi: 10.1117/1.JBO.21.2.025007

Fig. 8.

Fig. 8

Experimental results from biological cells: (a) representative bright-field reflectance white-light image of a cell; (b) representative height map obtained by AFM from the same cell (EGFR-knockdown variant shown here), scale bar 5  μm; (c) tail-fitting of experimentally obtained Fourier transform of reflectance intensity averaged throughout the cell area of interest; red dot corresponds to the predicted thickness, where Σ˜L is estimated and the green dot corresponds to the midsample depth, where Σ˜R is estimated for this particular spectrum. (d) Match between the cell thickness evaluated from the frequency content of its reflectance intensity compared to that obtained with AFM; horizontal error bars indicate the range (standard deviation) of thicknesses within the sample as measured by AFM.