Table 2.

Performance features of qPCR assays.

Assay	Taxonomical affiliates	GenBank accession no.	Size (bp)	Slope	Intercept	qPCR efficiency	Range of quantification	Limit of detection (plasmid copies)a
Av4143	Lactobacillus	LN864462	244	3.75	44.05	84.7	25 – 2.5E8	6
Av163	Gallibacterium	LN864463	74	3.3	40.9	100	18 – 3.6E8	9
Av43	Firmicutes	LN864464	100	3.39	41.4	97	25 – 2.5E8	6
Av216	Other bacteria	LN864465	122	3.4	40.5	96.8	24 – 2.4E8	6
Av24	Fusobacteriaceae	LN864466	76	3.4	40.1	96.8	20 – 2.0E8	5
Av13	Fusobacteriaceae	LN864467	119	3.43	42.3	95.6	19 – 3.8E8	8

^aDefined as 90% positive detection of qPCR.