FIGURE 3.

ech2-2 is the causative mutation that suppresses CCE in a fugu5-1 background. (A) Cotyledons from plants grown on rockwool were collected, fixed at 25 DAS and cleared for microscopic analysis. The number and size of palisade cells in the subepidermal layer were determined in the wild type and the indicated mutants. Cell numbers are mean ± standard deviation (n = 8). The cell size values are mean cell areas of 160 cells from eight different cotyledons. (B) Cotyledons from plants grown on MS media supplemented with 2% sucrose were collected, fixed at 21 days after seed germination and cleared for microscopic analysis. The number and size of palisade cells in the subepidermal layer were determined as described in (A). NS, not significant.