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. 2016 Feb 17;7:97. doi: 10.3389/fpls.2016.00097

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Copyright © 2016 Petre, Hecker, Germain, Tsan, Sklenar, Pelletier, Séguin, Duplessis and Rouhier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RISP interacts with M. larici-populina urediniospores in vitro. Recombinant His-RISP, RISP-GFP-His, GFP and Neisseria meningitidis Grx were included in a pull-down assay with M. larici-populina urediniospores (isolate 98AG31). Recombinant proteins from the different fractions of the pull-down assay were visualized on 15% SDS-PAGE. Input: protein solution before centrifugation. Pull-down supernatant: supernatant after the first centrifugation. Washing supernatant: supernatant after the second centrifugation for washing. Elution: supernatant after incubation at 95°C in Laemmli buffer.