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. 2016 Feb 17;7:97. doi: 10.3389/fpls.2016.00097

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Copyright © 2016 Petre, Hecker, Germain, Tsan, Sklenar, Pelletier, Séguin, Duplessis and Rouhier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RISP inhibits M. larici-populina growth on poplar leaves. (A) The left part of a Beaupré leaf was inoculated with M. larici-populina urediniospores (isolate 98AG31, virulent on Beaupré) mixed with TE pH 8.0 buffer, whereas the right part was inoculated with the same batch of urediniospores mixed with 100 μM recombinant RISP. Pictures were taken at 10 dpi. (B) Selected areas of a Beaupré leaf were treated with 1 mL of ddHOH, TE pH 8.0 buffer or 100 μM RISP, and then the whole leaf was immediately inoculated by pulverization with M. larici-populina urediniospores (isolate 98AG31). Pictures were taken just after inoculation as well as 1, 2, or 3 weeks post-inoculation (wpi).