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. 2016 Feb 17;7:97. doi: 10.3389/fpls.2016.00097

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Copyright © 2016 Petre, Hecker, Germain, Tsan, Sklenar, Pelletier, Séguin, Duplessis and Rouhier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RISP induces poplar cell culture alkalinisation. (A) His-RISP was added to poplar cell suspensions (P. trichocarpa x P. deltoides H11-11 hybrid) at a concentration of 1 μM and the pH was recorded at regular times as indicated. A control without protein was performed with TE pH 8.0 buffer. Error bars: SE, n = 3. (B) His-RISP was added to the H11-11 cell suspensions at concentrations ranging from 0.1 nM to 1 μM and the pH was recorded after 30 min. Error bars: SE, n = 3. TE buffer values (stable at pH 4.2 during the experiment) were used to calculate the pH ratio for each time-point.