Abstract
The adenylytransferase/acetyltransferase methods of gentamicin assay have been evaluated for accuracy, speed, and cost. For a comparable cost of materials the latter method is more accurate than that using the adenylytransferase enzyme. The acetyltransferase method is much quicker than the adenyltransferase due to the shorter time necessary for radioactive counting. Sonication is an easier method of enzyme preparation than the previously used osmotic shock technique. The acetyltransferase method is reproducible and there was a very good correlation between it and a microbiological agar-plate diffusion method.
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Selected References
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- Bennett J. V., Brodie J. L., Benner E. J., Kirby W. M. Simplified, accurate method for antibiotic assay of clinical specimens. Appl Microbiol. 1966 Mar;14(2):170–177. doi: 10.1128/am.14.2.170-177.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benveniste R., Davies J. R-factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics. FEBS Lett. 1971 May 20;14(5):293–296. doi: 10.1016/0014-5793(71)80282-x. [DOI] [PubMed] [Google Scholar]
- Furger F., Russi A., Kayser F. H. Schnellmethoden zur quantitativen Bestimmung von Aminoglykosid-Antibiotika. Schweiz Med Wochenschr. 1973 May 26;103(21):779–782. [PubMed] [Google Scholar]
- Haas M. J., Davies J. Enzymatic acetylation as a means of determining serum aminoglycoside concentrations. Antimicrob Agents Chemother. 1973 Oct;4(4):497–499. doi: 10.1128/aac.4.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Noone P., Pattison J. R., Samson D. Simple, rapid method for assay of aminoglycoside antibiotics. Lancet. 1971 Jul 3;2(7714):16–19. doi: 10.1016/s0140-6736(71)90007-9. [DOI] [PubMed] [Google Scholar]
- Phillips I., Warren C., Smith S. E. Serum gentamicin assay: a comparison and assessment of different methods. J Clin Pathol. 1974 Jun;27(6):447–451. doi: 10.1136/jcp.27.6.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reeves D. S. Assay of gentamicin. Lancet. 1972 Dec 23;2(7791):1369–1370. doi: 10.1016/s0140-6736(72)92816-4. [DOI] [PubMed] [Google Scholar]
- Smith D. H., Van Otto B., Smith A. L. A rapid chemical assay for gentamicin. N Engl J Med. 1972 Mar 16;286(11):583–586. doi: 10.1056/NEJM197203162861106. [DOI] [PubMed] [Google Scholar]
- ten Krooden E., Darrell J. H. Rapid gentamicin assay by enzymatic adenylylation. J Clin Pathol. 1974 Jun;27(6):452–456. doi: 10.1136/jcp.27.6.452. [DOI] [PMC free article] [PubMed] [Google Scholar]