Table 6.
DGGE bands sequenced, band length, associated GenBank accession numbers, and phylogenetic affiliation from profiles of fungal populations.
| Phylotype | Phylogenetic affiliation | ||||
|---|---|---|---|---|---|
| Band codea | Sequence length (bp) | Accession code | Taxonomic linageb(Phylum, Class, Order, Family, Genus) | Closest matchc(accession no.) | Similarity (%)d |
| F1 | 231 | KT991592 | Ascomycota (100), Pezizomycetes (100), Pezizales (100), Pyronemataceae (100), Pseudaleuria (100) | Uncultured fungus clone (JF432996) | 100 |
| F2 | 219 | KT991593 | Ascomycota (100), Pezizomycetes (98), unclassified_Pezizomycetes_order (69) | Uncultured fungus clone (JX323746) | 97.72 |
| F3 | 173 | KT991594 | Ascomycota (100), Pezizomycetes (99), unclassified_Pezizomycetes_order (75) | Uncultured fungus clone (JX323746) | 97.70 |
| F4 | 189 | KT991595 | Ascomycota (100), Sordariomycetes (100), Hypocreales (99), Hypocreales_family_incertae_sedis (60), Fusarium (60) | Fusarium equiseti (KM246255) | 98.4 |
| F5 | 165 | KT991596 | Ascomycota (100), Dothideomycetes (89), Pleosporales (89), Sporormiaceae (84), Preussia (74) | Uncultured fungus clone (JX340328) | 100 |
| F6 | 251 | KT991597 | Ascomycota (100), Eurotiomycetes (100), Onigenales (100), Arthrodermataceae (100), Ctenomyces (100) | Uncultured fungus clone (JX349691) | 99.2 |
| F7 | 186 | KT991598 | Fungi_phylum_incertae_sedis (100), Fungi_class_incertae_sedis (100), Mortierellales (87), Mortierellaceae (85), Mortierella (74) | Uncultured fungus clone (JX377362) | 97.85 |
| F8 | 192 | KT991599 | Fungi_phylum_incertae_sedis (100), Fungi_class_incertae_sedis (100), Mortierellales (99), Mortierellaceae (98), Mortierella (98) | Uncultured fungus clone (JX345268) | 99.48 |
| F9 | 169 | KT991600 | Ascomycota (100), Dothideomycetes (100), Capnodiales (100), Capnodiales_family_incertae_sedis (100), Cladosporium (100) | Graphiopsis chlorocephala (JN116693) | 100 |
| F10 | 180 | KT991601 | Fungi_phylum_incertae_sedis (100), Fungi_class_incertae_sedis (100), Mortierellales (99), Mortierellaceae (94), Mortierella (94) | Uncultured fungus clone (JX387233) | 98.9 |
| F11 | 210 | KT991602 | Ascomycota (100), Sordariomycetes (100), Hypocreales (100), Hypocreales_family_incertae_sedis (100), Stachybotrys (100) | Uncultured Stachybotrys clone (KF493978) | 100 |
| F12 | 201 | KT991603 | Ascomycota (99), Sordariomycetes (96), Microascales (73), Microascaceae (73), Pseudallescheria (69) | Uncultured Pseudallescheria clone (KM108739) | 99.5 |
| F13 | 173 | KT991604 | Ascomycota (100), Sordariomycetes (98), Microascales (74), Microascaceae (74), Pseudallescheria (66) | Uncultured Pseudallescheria clone (KM108739) | 100 |
| F14 | 290 | KT991605 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (94), Psathyrella (60) | Uncultured fungus clone (GQ225128) | 100 |
| F15 | 292 | KT991606 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Psathyrella (64) | Uncultured fungus clone (GQ225128) | 100 |
| F16 | 249 | KT991607 | Ascomycota (87), Pezizomycetes (78), Pezizales (76), Pyronemataceae (74), Heydenia (50) | Pezizaceae sp (JQ775581) | 98.35 |
| F17 | 220 | KT991608 | Ascomycota (100), Pezizomycetes (96), Unclassified Pezizomycetes genus (53) | Uncultured fungus clone (JX323746) | 97.27 |
| F18 | 193 | KT991609 | Ascomycota (100), Sordariomycetes (100), Microascales (75), Microascaceae (75), Pseudallescheria (71) | Uncultured Pseudallescheria clone (KM108739) | 100 |
| F19 | 193 | KT991610 | Ascomycota (100), Sordariomycetes (97), Microascales (81), Microascaceae (80), Pseudallescheria (79) | Uncultured Pseudallescheria clone (KM108739) | 100 |
| F20 | 212 | KT991611 | Ascomycota (100), Sordariomycetes (100), Hypocreales (100), Hypocreales_family_incertae_sedis (100), Stachybotrys (100) | Uncultured fungus clone (JX348029) | 100 |
| F21 | 202 | KT991612 | Ascomycota (100), Sordariomycetes (100), Hypocreales (100), Hypocreales_family_incertae_sedis (93), Fusarium (93) | Fusarium equiseti isolate (KM246255) | 100 |
| F22 | 194 | KT991613 | Ascomycota (100), Sordariomycetes (99), Hypocreales (99) Hypocreales_family_incertae_sedis (84), Fusarium (83) | Uncultured Fusarium clone (KP235758) | 97.94 |
| F23 | 193 | KT991614 | Ascomycota (100), Sordariomycetes (98), Microascales (85), Microascaceae (85), Pseudallescheria (82) | Uncultured Pseudallescheria clone (KM108739) | 100 |
| F24 | 221 | KT991615 | Fungi_phylum_incertae_sedis (100), Fungi_class_incertae_sedis (100), Mortierellales (100), Mortierellaceae (99), Mortierella (99) | Uncultured fungus clone (GQ866183) | 97.26 |
| F25 | 305 | KT991616 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Hormographiella aspergillata (KP132299) | 100 |
| F26 | 304 | KT991617 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Uncultured Coprinopsis clone (GQ219811) | 100 |
| F27 | 298 | KT991618 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (99), Psathyrella (61) | Uncultured fungus clone (GQ225128) | 100 |
| F28 | 287 | KT991619 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Uncultured Coprinopsis clone (GQ219811) | 100 |
| F29 | 307 | KT991620 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Hormographiella aspergillata (KP132299) | 100 |
| F30 | 204 | KT991621 | Ascomycota (100) Sordariomycetes (100), Microascales (99), Microascaceae (99), Pseudallescheria (99) | Uncultured fungus clone (JX383001) | 94.2 |
| F31 | 204 | KT991622 | Ascomycota (100), Dothideomycetes (100), Capnodiales (100), Capnodiales_family_incertae_sedis (100), Davidiella (53) | Cladosporium sphaerospermum (KP174687) | 100 |
| F32 | 245 | KT991623 | Ascomycota (96), Sordariomycetes (90), Microascales (61), Microascaceae (60), Scedosporium (47) | Uncultured fungus clone (JQ989314) | 99.59 |
| F33 | 298 | KT991624 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (99), Psathyrella (58) | Uncultured fungus clone (GQ225128) | 100 |
| F34 | 297 | KT991625 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Hormographiella aspergillata (KP132299) | 100 |
| F35 | 308 | KT991626 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Uncultured Coprinopsis clone (GQ219811) | 100 |
| F36 | 305 | KT991627 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Hormographiella aspergillata (KP132299) | 100 |
| F37 | 279 | KT991628 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinopsis (100) | Coprinopsis sp. (AB499044) | 100 |
| F38 | 206 | KT991629 | Fungi_phylum_incertae_sedis (99), Fungi_class_incertae_sedis (99), Mortierellales (99), Mortierellaceae (95), Mortierella (95) | Uncultured soil fungus clone (JX489813) | 100 |
| F39 | 209 | KT991630 | Ascomycota (100), Sordariomycetes (100), Hypocreales (100), Hypocreales_family_incertae_sedis (95), Fusarium (95) | Fusarium equiseti isolate (KM246255) | 100 |
| F40 | 313 | KT991631 | Basidiomycota (100), Agaricomycetes (100), Agaricales (100), Psathyrellaceae (100), Coprinellus (100) | Uncultured fungus clone (JX353314) | 100 |
| F41 | 211 | KT991632 | Ascomycota (100), Sordariomycetes (100), Hypocreales (100), Hypocreales_family_incertae_sedis (100), Stachybotrys (100) | Uncultured Stachybotrys clone (KF493978) | 100 |
a
Band numbers correspond to those presented in Figure 3C for fungal samples.
b
Taxonomic string with bootstrap values (in parentheses), generated in mothur using Findley database.
c
Closest relative according to INSA (International Nucleotide Sequence Database).
d
Percentage sequence similarity with closest INSA using BLAST tool.