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. 2016 Feb 17;36(7):2119–2130. doi: 10.1523/JNEUROSCI.3056-15.2016

Figure 2.

Figure 2.

HDAC inhibitors block the reducing effect of repeated stress or CORT on AMPAR-EPSC. A, Input/output curves of AMPAR-EPSC evoked by a series of stimulus intensities in PFC pyramidal neurons from control (con) versus repeatedly stressed (RS) rats with the intraperitoneal injection of TSA (0.5 mg/kg, a pan-HDAC inhibitor) or vehicle. **p < 0.01, ANOVA. B, Dot plots showing the amplitude of AMPAR-EPSC evoked by the same stimulus in PFC pyramidal neurons from control versus stressed animals with intraperitoneal injections of TSA, SB (0.4 g/kg, a pan-HDAC inhibitor except for HDAC6), MGCD0103 (15 μg/kg, a selective inhibitor for HDAC1 and HDAC2), or MS-275 (15 μg/kg, a selective inhibitor for HDAC1). Inset, Representative AMPAR-EPSC traces. Scale bars, 50 pA, 20 ms. C, Immunoblots and quantification analysis of the level of acetylated H3 and total H3 in cortical slices either from rats injected intraperitoneally with various agents (vehicle, 15 μg/kg MGCD0103, or 15 μg/kg or 5 mg/kg MS-275) or treated with vehicle versus MS-275 (10 μm, 4 h in vitro). **p < 0.01, *p < 0.05, t test. D, Bar graphs showing the mEPSC amplitude and frequency in cultured cortical neurons treated with CORT (100 nm, 7 d) in the absence or presence of TSA (1 μm), SB (500 μm), tubastatin A (10 μm, an inhibitor for HDAC6), MGCD0103 (500 nm), or MS-275 (600 nm). **p < 0.01, *p < 0.05, ANOVA. Inset, Representative mEPSC traces. Scale bar, 20 pA, 5 s.