Figure 7.

mGlu5^R/R mice have impaired sensorimotor gating, reduced nonsocial anxiety, and an antidepressant phenotype. A, PPI of startle amplitude, measured by the percent inhibition of the startle to 110 dB tone when preceded by a 90 dB prepulse, is reduced in mGlu5^R/R mice compared with WT controls (N = 20, N = 16, and N = 13 for WT, Het, and mGlu5^R/R littermates, respectively). Prepulse effect: F_(1,43) = 117.48, p < 0.0001; genotype × prepulse: F_(2,43) = 4.52, p = 0.02. Startle amplitude at 74 or 90 dB was not different between genotypes. Data are mean ± SEM percent inhibition of the 110 dB startle amplitude by the 74 dB and the 90 dB prepulse intensities. *p < 0.05 versus WT (LSD post hoc tests). **p < 0.01. B, Open field activity, measured as a ratio of the distance traveled in the center to total distance traveled in an open arena, was increased in mGlu5^R/R mice compared with WT or Het mice (F_(2,82) = 5.376, p = 0.006; Dunnet's post hoc tests) (N = 19, N = 44, and N = 22 for WT, Het, and mGlu5^R/R littermates, respectively). C, mGlu5^R/R mice spent more time in the open arms as measured by seconds or percentage of total time in the maze (N = 15, N = 22, and N = 17 for WT, Het, and mGlu5^R/R littermates, respectively). Sex effect: F_(1,48) = 6.14, p = 0.02; genotype effect: F_(2,48) = 3.45, p = 0.04; interaction: p = 0.21. D, Number of entries into closed or open arms of an elevated plus maze were not different between genotypes. E, The latency to first float in the Porsolt Swim Test was not different among genotypes. F, The percentage of incidence of floating as scored in the first and second swim sessions was reduced in mGlu5^R/R mice (N = 15, N = 22, and N = 17 for WT, HET, and mGlu5^R/R littermates, respectively). Genotype effect: F_(2,49) = 5.52, p = 0.007; no interactions with the genotype factor: p > 0.20.