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. 2016 Feb 17;36(7):2247–2260. doi: 10.1523/JNEUROSCI.2528-15.2016

Figure 7.

Figure 7.

Behavioral characterization of Sorbs2 KO mice. A, Locomotion activity in open-field test and (B) anxiety level in elevated-zero maze test were similar between Sorbs2 KO and WT mice. C, Sorbs2 KO mice showed dramatically reduced response to stimuli in the acoustic startle response test (two-way ANOVA, startle stimulus volume × genotype: interaction, p < 0.0001; effect of genotype, p < 0.0001; effect of startle stimulus volume, p < 0.0001; Bonferroni post hoc test: ***p < 0.0001; WT: n = 13, KO: n = 12). D, PPI is significantly impaired in Sorbs2 KO mice (two-way ANOVA, prepulse stimulus volume × genotype: interaction, p < 0.0001; effect of genotype, p = 0.0677; effect of prepulse stimulus volume, p < 0.0001; Bonferroni post hoc test: **p < 0.01, ***p < 0.0001). E, Scheme of novel object recognition (NOR) test. Two identical objects (T1 and T2) were used in the training session, and two different novel objects N1 or N2 were exposed to mice in the test session 10 min or 24 h after training to assess short-term (10 min) or long-term (24 h) object recognition memory, respectively. F, Short-term object recognition memory is intact in Sorbs2 KO mice (WT: 67.21 ± 2.51%, n = 15; KO: 66.56 ± 2.43%, n = 16; two-tailed t test, p = 0.8547). G, Long-term object recognition memory is impaired in Sorbs2 KO mice (WT: 64.04 ± 3.54%, n = 15; KO: 54.52 ± 2.93%, n = 16; two-tailed t test, *p < 0.05). H, Sorbs2 KO mice behaved similarly as WT during the training of contextual fear conditioning (CFC) task. Percentages of freezing time per 30 s bin across the training were compared between genotypes. Arrows indicate the delivery of footshocks. I, Sorbs2 KO mice showed reduced freezing time in CFC test (WT: 33.41 ± 3.04%, n = 15; KO: 21.61 ± 3.35%, n = 14; two-tailed t test, *p < 0.05). J, K, WT and Sorbs2 KO mice behaved similarly during the training (J) and the memory test (K) of toned fear conditioning (TFC) task. B, Baseline; T1–T5 indicate the presence of tones; ITI, intertrial interval. Arrows indicate the delivery of footshocks. L, M, Sorbs2 KO mice showed normal spatial learning and memory as WT mice in Morris water maze test. T, Target quadrant; L, adjacent left quadrant; R, adjacent right quadrant; O, opposite quadrant. Error bars indicate SEM.