Figure 1.

Soybean leaf abscission system and the overview of transcriptome changes in abscission. (A) Stem-petiole explants were prepared by cutting the leaf blade off leaving an ~5-mm triangular portion of the leaf blade still attached to the petiole and cutting the stem at ~4 cm below the node (dashed line). (B) Explants were treated with 25 μL L⁻¹ ethylene in air saturated with water. The upper AZ (LAZ) and Non-AZ (NAZ, petiole) were collected at 0, 12, 24, 48, and 72 h. (C) Percentile of soybean leaf abscission and plots of RNA-seq results for a constitutive ubiquitin gene UBI3a (Glyma20g27950) and two abscission specific marker genes, CEL01 (Glyma11g02350) and PG-840 (Glyma20g02840). (D) Summary of soybean transcription factors (TFs) differentially regulated more than eight-fold (log₂ > 3 or < −3, p < 0.015) (E) Summary of differentially expressed genes (DEGs) in the entire transcriptome that were differentially expressed more than eight-fold (log₂ >3 or < −3, p < 0.015).