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. 2016 Feb 17;7:105. doi: 10.3389/fpls.2016.00105

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Copyright © 2016 Bielczynski, Schansker and Croce.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2D-PAGE of thylakoids isolated from plants grown under different light intensities. (A) The BN-PAGE of thylakoids isolated from plants grown under GL200, 600, and 1800, solubilized with 1% α-DDM. (B) Sum of nine 2D-PAGE gels of isolated thylakoids from plants grown under all light conditions. The red contours represent ROIs of selected proteins in specific complexes. The blue contours represent the integrated areas for quantification of a selected protein independent of the complex. On the top, a lane from the BN-PAGE is shown. (C) Overlap of the gels of thylakoids from plants grown under GL200, 600, and 1800, that are shown in the red, green, and blue channels, respectively. (D) 3D surface plots of CP43 + 47 and D1 megacomplex ROIs, respectively.