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. 2016 Feb 17;7:105. doi: 10.3389/fpls.2016.00105

Table 1.

Pigment analysis of plants grown under different light conditions.

GL200 GL600 GL1800
Chls/fresh weight [mg/g] 0.8621 ± 0.1156 0.7821 ± 0.0354 0.3893 ± 0.0986
Chl a/b 3.235 ± 0.022 3.249 ± 0.006 3.562 ± 0.047
Chl/Car 3.905 ± 0.024 3.698 ± 0.015 3.228 ± 0.054
Neo/100 Chls 3.424 ± 0.048 3.743 ± 0.031 4.004 ± 0.178
Vio/100 Chls 2.753 ± 0.033 3.035 ± 0.005 4.106 ± 0.183
Ant/100 Chls 0.196 ± 0.023 0.381 ± 0.010 0.621 ± 0.030
Lut/100 Chls 12.364 ± 0.0125 12.900 ± 0.041 13.981 ± 0.284
Zea/100 Chls 0.195 ± 0.005 0.296 ± 0.027 0.678 ± 0.018
β-Car/100 Chls 6.677 ± 0.017 6.688 ± 0.069 7.592 ± 0.176
Cars/100 Chls 25.609 ± 0.157 27.044 ± 0.108 30.98 ± 0.513
(Z + 0.5 × A)/(Z + A + V) 0.0932 ± 0.002 0.131 ± 0.007 0.183 ± 0.003

Total chlorophylls (Chls) were quantified by fitting the 80% acetone extracts from a leaf from five different plants (n = 5) grown under GL200, 600, and 1800 and normalized to the fresh weight. The chlorophyll a/b (Chl a/b) ratio and chlorophyll/carotenoid (Chl/Car) ratio was determined in the same way from isolated thylakoid membranes in three repetitions (n = 3). The same extracts were used for the quantification by HPLC of the carotenoids: neoxanthin (Neo), violaxanthin (Vio), anteraxanthin (Ant), zeaxanthin (Zea), lutein (Lut) and β-carotene (β-car). All carotenoids were calculated per 100 Chls.