Table 1.
GL200 | GL600 | GL1800 | |
---|---|---|---|
Chls/fresh weight [mg/g] | 0.8621 ± 0.1156 | 0.7821 ± 0.0354 | 0.3893 ± 0.0986 |
Chl a/b | 3.235 ± 0.022 | 3.249 ± 0.006 | 3.562 ± 0.047 |
Chl/Car | 3.905 ± 0.024 | 3.698 ± 0.015 | 3.228 ± 0.054 |
Neo/100 Chls | 3.424 ± 0.048 | 3.743 ± 0.031 | 4.004 ± 0.178 |
Vio/100 Chls | 2.753 ± 0.033 | 3.035 ± 0.005 | 4.106 ± 0.183 |
Ant/100 Chls | 0.196 ± 0.023 | 0.381 ± 0.010 | 0.621 ± 0.030 |
Lut/100 Chls | 12.364 ± 0.0125 | 12.900 ± 0.041 | 13.981 ± 0.284 |
Zea/100 Chls | 0.195 ± 0.005 | 0.296 ± 0.027 | 0.678 ± 0.018 |
β-Car/100 Chls | 6.677 ± 0.017 | 6.688 ± 0.069 | 7.592 ± 0.176 |
Cars/100 Chls | 25.609 ± 0.157 | 27.044 ± 0.108 | 30.98 ± 0.513 |
(Z + 0.5 × A)/(Z + A + V) | 0.0932 ± 0.002 | 0.131 ± 0.007 | 0.183 ± 0.003 |
Total chlorophylls (Chls) were quantified by fitting the 80% acetone extracts from a leaf from five different plants (n = 5) grown under GL200, 600, and 1800 and normalized to the fresh weight. The chlorophyll a/b (Chl a/b) ratio and chlorophyll/carotenoid (Chl/Car) ratio was determined in the same way from isolated thylakoid membranes in three repetitions (n = 3). The same extracts were used for the quantification by HPLC of the carotenoids: neoxanthin (Neo), violaxanthin (Vio), anteraxanthin (Ant), zeaxanthin (Zea), lutein (Lut) and β-carotene (β-car). All carotenoids were calculated per 100 Chls.